The way Navigation Pro is coded, doesn’t let you change the layout on single posts because of this line of PHP code located around line 31 in image-functions.php.
What happens if we remove the code which checks to make sure the layout used is full width:
genesis_site_layout() === 'full-width-content' &&
Then you get this :
Resulting in this :
You can then change the layout by adding this line of code to the Navigation Pro themes single.php template file :
add_filter( 'genesis_pre_get_option_site_layout', '__genesis_return_content_sidebar' );